Table 1 Primers and Mg2+ concentrations for PCR amplification and pyrosequencing
From: Frequencies of genetic polymorphisms related to triptans metabolism in chronic migraine
SNPs | Forward primer (5′–3′) | Reverse primer (5′–3′) | Sequencing primer (5′–3′) | Mg2+ concentration (mM) |
|---|---|---|---|---|
CYP1A2*1C | CTTCTTGGATGCTTATGA | aTGTAATTCCAGCTACTCG | ACCGCAACCTCCGCC | 1 |
CYP1A2*1F | aAGTGGAAACTGAGATGAT | ATACCAGAAAGACTAAGC | CTACCATGCGTCCTG | 1 |
CYP3A4*1B | GGGATGAATTTCAAGTAT | aGGGTTCTTATCAGAAACT | CAGCCATAGAGACAAGG | 1 |
GNB3 C825T | ACGAGAGCATCATCTG | aATGGAGTCCCAGACAT | ATCTGCGGCATCACG | 1.5 |