Fig. 2

Acute glyceryl trinitrate (GTN) administration induces delayed occurrence of anxiety-like behaviors. A Representative traces of mice in 10-min Elevated Plus Maze (EPM) test at 2 h, 4 h, and 24 h after VEH or GTN injection. B-E Time spent in the open arms (B), the number of open arm entries (C), time spent in the closed arms (D), and the number of closed-arm entries (E) during the EPM test at 2 h (n = 8 mice per group), 4 h (n = 9 mice per group), and 24 h (n = 9 mice per group) after VEH or GTN injection. Significance was assessed by a two-tailed unpaired Student’s t-test. All data are presented as the mean ± S.E.M. *P < 0.05, **P < 0.01