Fig. 7
Peripheral TAAR1 contributes to mechanical pain hypersensitivity in mice. a Escape threshold after intra-TG injection of vehicle or tyramine at 0.1 nmol, 1 nmol or 5 nmol. *p < 0.05 vs. vehicle at the corresponding time point, two-way ANOVA. BL, baseline. b Intra-TG pre-injection of EPPTB (3 nmol) prevented 1 nmol tyramine-induced mechanical hypersensitivity. *p < 0.05 vs. vehicle at 1 h, one-way ANOVA. c, Effects of PKCθ-siRNA or the control siRNA (NC-siRNA) on tyramine (1 nmol, intra-TG injection; arrow)-induced mechanical hypersensitivity of acute pain. *p < 0.05 vs. NC-siRNA at the corresponding time point, #p < 0.05 vs. NC-siRNA at 0 h, two-way ANOVA. d Intra-TG pre-injection of Kv1.4-siRNA occluded 1 nmol tyramine (arrow)-induced mechanical hypersensitivity. *p < 0.05 vs. NC-siRNA at 0 h, #p < 0.05 vs. NC-siRNA at 0 h, two-way ANOVA. e Escape threshold to mechanical stimuli in the sham- and ES-operated groups. **p < 0.01 vs. sham, two-way ANOVA. f Western blot analysis of TAAR1 protein abundance in the sham- and ES (day 5)-operated groups. *p < 0.05 vs. sham, unpaired t test. The immunoblots are representative of the results of at least three independent experiments. g Intra-TG injection of EPPTB (3 nmol, arrow) alleviated mechanical allodynia on day 5 post-ES. *p < 0.05, **p < 0.01 vs. vehicle at the corresponding time point, two-way ANOVA. H, Intra-TG injection of lenti-Kv1.4-up occluded the EPPTB (3 nmol, arrow)-induced alleviation of mechanical allodynia in ES 5 d mice. *p < 0.05 vs. ES 5 d, #p < 0.05 vs. lenti-NC-up at 1 h, two-way ANOVA. At least 8 mice were used per experimental group in all behavior experiments