Fig. 8

Regulation of central sensitization by ZD 7288 in CM rats. A Representative western blots of CGRP and c-Fos. B and C The protein levels of CGRP and c-Fos were significantly elevated in the CM group compared with those in the Sham group, and there was no statistical difference between the CM and CM + Vehicle groups. The expression levels of CGRP and c-Fos in the CM + ZD 7288 group drastically reduced compared to those in the CM + Vehicle group. (n = 6/group, **p < 0.01, N, S, not significant). D-F Immunofluorescence staining of c-Fos, CGRP, and SP in the PAG. G-I The number of c-Fos-positive cells and the average fluorescence intensity of CGRP and SP in the CM group were substantially increased compared to those in the Sham group, and there was no significant change between the CM and CM + Vehicle groups. The number of c-Fos-positive cells and the average fluorescence intensity of CGRP and SP in the CM + ZD 7288 group were visibly lower than those in the CM + Vehicle group. (n = 6/group, **p < 0.01, N,S, not significant, scale bar = 20 μm)