Skip to main content
Fig. 6 | The Journal of Headache and Pain

Fig. 6

From: Activation of CB1R alleviates central sensitization by regulating HCN2-pNR2B signaling in a chronic migraine rat model

Fig. 6

Regulation on central sensitization by NE in CM rats. A Representative western blots of CGRP and c-Fos. B and C. The protein levels of CGRP and c-Fos in the CM group were significantly higher than those in the Sham group, and there was no significant difference between the CM group and CM + Vehicle group. The CGRP and c-Fos expression levels in the CM + NE group were sharply reduced compared with those in the CM + Vehicle group. (n = 6/group, **p < 0.01, N, S, not significant). D-F Immunofluorescence staining of c-Fos, CGRP, and SP in the PAG. G-I The number of c-Fos-positive cells and the average fluorescence intensity of CGRP and SP were substantially higher in the CM group than those in the Sham group, and there was no statistical difference between the CM and CM + Vehicle groups. Compared to the CM + Vehicle group. The number of c-Fos-positive cells and the average fluorescence intensity of CGRP and SP were materially decreased in the CM + NE group. (n = 6/group, **p < 0.01, N,S, not significant, scale bar = 20 μm)

Back to article page