Fig. 10
The influence of AM 251 and ZD 7288 on the expression levels of pNR2B, CGRP, and c-Fos in CM rats. A Representative western blots of pNR2B and CGRP. B and C The protein levels of pNR2B and CGRP were profoundly improved in the CM group compared with those in the Sham group, and there was no substantial difference between the CM and CM + Vehicle groups. Compared to the CM group, the levels of pNR2B and CGRP in the CM + AM 251 group were further increased. However, the expression of pNR2B and CGRP in the CM + AM 251 + ZD 7288 group was materially decreased compared to the CM + AM 251 group. (n = 6/group, *p < 0.05, **p < 0.01, N,S, not significant, scale bar = 20 μm). D and E The average fluorescence intensity of CGRP and the number of c-Fos-positive cells were statistically higher in the CM group than those in the Sham group. Compared with the CM group, the average fluorescence intensity of CGRP and the number of c-Fos-positive cells were visibly increased in the CM + AM 251 group. Importantly, the average fluorescence intensity of CGRP and the number of c-Fos-positive cells in the CM + AM 251 + ZD 7288 group sharply declined compared to the CM + AM 251 group. (n = 6/group, *p < 0.05, **p < 0.01, N,S, not significant, scale bar = 20 μm). F-G Immunofluorescence staining of CGRP and c-Fos in the PAG