Table 2 Overview of study compounds used in vivo (alphabetical order)

GTN

Cambrex (Germany) via RegionH Pharmacy (Denmark)

Nitric oxide (NO) donor

10 mg/kg, i.p

2 h prior to test

12.2% alcohol in saline

Levcromakalim

Tocris, Bio-Techne Ltd (UK)

K_ATP channel opener

1 mg/kg, i.p

2 h prior to test

2% DMSO in saline

Anti-PACAP mAb

H. Lundbeck (Denmark)

Human anti-PACAP mAb

30 mg/kg, i.p

24 h before first test (pre-treatment)

IgG control mAb in vehicle provided by Lundbeck

Midazolam

Hameln Pharma GMBH (Germany) via RegionH Pharmacy (Denmark

Benzodiazepine

2 mg/kg, i.p

10 min prior to test

Saline

PACAP38

CASLO ApS (Denmark)

PAC₁-. VPAC_1-2 receptor agonists

2 μg/kg, s.c

1 h prior to test

Saline

Sumatriptan

GlaxoSmithKline (Denmark) via Region Hovedstaden Pharmacy (Denmark

5-HT_1B/1D/1F receptor agonist

0.6 mg/kg, i.p

20 min prior to PACAP38 injection

Saline