Fig. 3

GTN signaling is independent from PACAP. Data is shown as square root transformed (SQRT)50% withdrawal threshold (g). A Responses two hours after GTN (10 mg/kg, i.p.) or vehicle injection (i.p.) in wildtype mice (n = 12 per group) on five test days. Anti-PACAP antibody or IgG control (30 mg/kg, i.p.) was given on day 0. Two-way ANOVA with Tukey’s post hoc test for multiple comparisons was used with data represented as means ± SEMs. * = P < 0.05, ** = P < 0.01, *** = P < 0.001, and **** = P < 0.0001. B Descriptive visualization of individual data points for baseline and day 9 of the curves on the left-hand side (Fig. 3A) with mean bars and SEMs