Fig. 2

Anti-PACAP antibody block PACAP38-induced hypersensitivity. Data are shown as square root transformed (SQRT) 50% withdrawal threshold (g). A Responses one hour after PACAP38 (2 µg/kg, s.c.) or vehicle injection (s.c.) in wildtype mice (n = 12 per group) on five test days. Mice were pre-treated with anti-PACAP antibody or IgG control (30 mg/kg, i.p.) on day 0. Two-way ANOVA with Tukey’s post hoc test for multiple comparisons was used with data represented as means ± SEMs. * = P < 0.05, ** = P < 0.01, *** = P < 0.001, and **** = P < 0.0001. B Descriptive visualization of individual data points for baseline and day 9 of the curves on the left-hand side (Fig. 2A) with mean bars and SEMs