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Fig. 3 | The Journal of Headache and Pain

Fig. 3

From: Exploring the neurobiology of the premonitory phase of migraine preclinically – a role for hypothalamic kappa opioid receptors?

Fig. 3

Chemogenetic manipulation of KORCRE neurons in the ARC increased water consumption and urination in both female and male mice. KORCRE or KORWT female and male mice were individually placed in metabolic chambers and received a single dose of CNO (DREADD specific agonist; 5 mg/kg, i.p.) four weeks after stereotaxic administration of AAV8-hSyn-DIO-hM4D(Gi)-mCherry virus (100 nL) into the right ARC. A Water consumption and B volume of urine, as outcome measurements of thirst and polyuria, respectively, were evaluated for 120 min after treatment. C Linear regression was performed to evaluate the correlation between water consumption and urination in KOR.CRE mice. Female and male data were combined. Data are presented as mean ± SEM and analyzed using one-way ANOVA followed by Sidak’s multiple comparison test (A) and (B) with * representing p < 0.05 compared to the control group (n = 10 – 13; Panel A: 5 females/6 males WT and 6 females/7 males HET; Panels B and C: 5 females/5 males WT and 6 females/7 males HET)

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