Fig. 8From: A c-Fos activation map in nitroglycerin/levcromakalim-induced models of migrainec-Fos activation map of brain stem structures in the LEV-induced chronic migraine model. A. Representative pictures of c-Fos (red) and NeuN (green) immunofluorescence labeling in the vlPAG. Scale bars =100 μm. B-C. In vlPAG, no significant differences in c-Fos+ cell density (B) and the percentage of c-Fos + cells in NeuN+ cells (C) were found. n = 5/group. D. Representative pictures of c-Fos (red) and NeuN (green) immunofluorescence labeling in the Sp5c. Scale bars =50 μm. E–F. Sp5c showed a significant increase of c-Fos+ cell density (E) and the percentage of c-Fos+ cells in NeuN+ cells (F), alleviated by OLC. n = 5/group. One-way ANOVA with the Tukey post hoc tests, * P < 0.05, **P < 0.01. Abbreviations: vlPAG, ventrolateral periaqueductal gray; OLC, olcegepant; Sp5c, caudal part of the spinal trigeminal nucleus; LEV, levcromakalimBack to article page