Table 9 Common targets, with similar changes in mRNA- and small RNA sequencing data for validation of predictions at mRNA level in ictal vs interictal comparison. Description and pain phenotype matches based on Human Pain Gene [53], Pain Research Forum [54], DisGeNET [55], and GeneCards [56], complemented with other literature data. logFC: logarithm of the fold change of measured mRNA data
Ictal vs Interictal | ||||
|---|---|---|---|---|
Target | logFC | Node strength | Description | Pain/inflammation related disease/processes (if applicable) |
RAB3B | -1.370 | 3 | RAB3B, member RAS oncogene family | |
LRRTM2 | -1.051 | 2 | leucine rich repeat transmembrane neuronal 2 | excitatory synaptic transmission [80], AMPA receptor transmission [81], |
NOX5 | -1.051 | 1 | NADPH oxidase 5 | oxidative stress [82] |
FAT3 | -1.003 | 1 | FAT atypical cadherin 3 | neuropathy [55] |
CBARP | -0.955 | 1 | CACN subunit beta associated regulatory protein | Negatively regulates voltage-gated calcium channels [56] |
BEND6 | -0.890 | 1 | BEN domain containing 6 | epilepsy [66], inhibits Notch signalling in neural stem cells, thereby opposing their self-renewal and promoting neurogenesis [56] |
RPS27A | -0.675 | 1 | ribosomal protein S27a | microglia activation in neurodegenrative diseases [83], upregulated in enhanced pain sensitivity [62] |
TBCA | -0.639 | 1 | tubulin folding cofactor A | brain injury, ischemia [66] |
FRG1B | -0.620 | 1 | FSHD region gene 1 family member B, pseudogene | |
ZNF730 | 1.175 | -1 | zinc finger protein 730 | transcriptional regulation [56] |
ZNF704 | 0.967 | -1 | zinc finger protein 704 | transcription factor [56], potential candidate gene for aging in women [84] |
MKI67 | 0.826 | -1 | marker of proliferation Ki-67 | cellular proliferation [56] |