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Fig. 7 | The Journal of Headache and Pain

Fig. 7

From: HMGB1 in the mPFC governs comorbid anxiety in neuropathic pain

Fig. 7

Systemic anti-HMGB1 mAb reversed the hyperexcitability of pyramidal neurons in mPFC layer 2/3 after p-IONX. a Schedule of experimental procedures. Anti-HMGB1 mAb was administered intraperitoneally. b Example image of whole cell patch-clamp recordings on mPFC layer 2/3 pyramidal neurons. Scale bar, 150 Î¼m. c Example traces of sEPSCs and sIPSCs in sham + saline, p-IONX + saline and p-IONX + anti-HMGB1 mAb groups. d, e Amplitude, frequency and charge transfer of sEPSCs (d) and sIPSCs (e). f Quantification of E/I ratio (∣CsEPSC∣/CsIPSC). g, h Resting potential (g) and rheobase (the lowest current that evoked action potential firing, h). i, j Representative traces of firing (i) and number of spikes (j) of action potentials evoked by injecting current at two-fold of rheobase. k Firing rate of action potentials evoked by step-current injection. l Autocorrelation and waveform (inset) of a representative glutamatergic neuron in in vivo single-unit recordings. m Mean frequency of spontaneous firing. *, ** and *** indicate P < 0.05, 0.01 and 0.001, respectively, compared with the indicated groups, unpaired t test or Mann–Whitney test. n = 9–10 neurons from 4 mice/group in (d-k) and n = 20 neurons from 8 mice/group in (m)

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