Fig. 3

Cellular and subcellular distribution of HMGB1 in the mPFC after p-IONX in MRL/MPJ mice. a-c Representative photomicrographs indicating the cellular distribution of HMGB1 in neurons (NeuN⁺, a), microglia (Iba1⁺, b) and astrocytes (S100b⁺, c) in the mPFC on D9 PO. DAPI was used to label nuclei. Scale bar, 50 μm. d Percentage of HMGB1-positive cells in neurons (NeuN⁺), microglia (Iba1⁺) and astrocytes (S100b⁺) on D9 PO in the mPFC. e Percentage of cells with HMGB1 cytoplasmic translocation in neurons (NeuN⁺), astrocytes (S100b⁺) and microglia (Iba1⁺) on D9 PO in the mPFC. f Quantification of Iba1 and S100b fluorescence intensity in the mPFC. g-h Representative images of western blot bands (g) and quantification of HMGB1 (h) in nuclear and cytoplasmic fractions from the mPFC on D9 PO. * and ** indicate P < 0.05 and 0.01, respectively, compared with the indicated groups, unpaired t test. n = 4/group