Fig. 2

HMGB1 expression in the mPFC was increased temporally in parallel to comorbid anxiety in neuropathic pain. a Schedule of experimental procedures. b Representative photomicrographs of HMGB1 immunostaining in the mPFC, BLA, vHPC and PBN. Scale bar, 200 μm. c Quantification of HMGB1 fluorescence intensity in the mPFC, BLA, vHPC and PBN on D9 PO. d, e Representative images of protein bands in western blotting (left) and quantification of HMGB1 expression (right) in the mPFC (d) and BLA (e) on D9 and D30 PO. *, ** and *** indicate P < 0.05, 0.01 and 0.001, respectively, compared with the indicated groups, ordinary one-way ANOVA with Tukey post hoc or Kruskal–Wallis test with Dunn’s post hoc in (c) and ordinary one-way ANOVA with Dunnett post hoc in (d) and (e). n = 4/group