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fig. 3 | The Journal of Headache and Pain

fig. 3

From: Cortical spreading depression can be triggered by sensory stimulation in primed wild type mouse brain: a mechanistic insight to migraine aura generation

fig. 3

Priming the barrel cortex with ouabain increased the incidence of whisker stimulation-induced CSDs. Each horizontal line represents continuous recording from one mouse primed with 0.1 mM ouabain and then subjected to whisker stimulation at 8–12 Hz for 10 min except a few that lasted a bit shorter or longer due to interventions needed (e.g. for clearing the airway) or timing errors in stimulator. Stimulation started after at least a 30-min silent period without any CSD. The number of ouabain-induced CSDs prior to starting stimulation is given before each line in parentheses. Stimulation (labeled blue) was stopped when a CSD was triggered (red circles). Green circles depict the occasional CSDs unrelated to stimulation. Bar graph shows that the CSD incidence was significantly higher (*p = 0.026, Chi-square test) during whisker stimulation compared to the non-stimulated periods. Only positive experiments are displayed on the left to simplify viewing of the flow charts by omitting the negative experiments (n = 6), but statistical analysis on the right included all animals regardless of the stimulation effectiveness (11 mice) and compared them to 16 non-stimulated mice treated with 0.1 mM ouabain. Because the CSD incidence was very low in controls, it was not suitable for illustration (i.e. 16 lines mostly with no incidences)

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