Fig. 7

Effects of the PI3K pathway inhibitor LY294002 on GLUA1 trafficking and central sensitization in CM rats. A, B, and C Pharmacological inhibition of the PI3K pathway significantly alleviated pain hypersensitivity in CM rats. Two-way ANOVA with the Bonferroni post hoc test; n = 6/group; **P < 0.01, ***P < 0.001 vs. the Sham + DMSO group; ##P < 0.01, ###P < 0.001 vs. the CM + DMSO group. D and E PI3K inhibition inhibited GLUA1 trafficking and reduced the p-GLUA1 level, which were increased in CM rats. C D and E Protein levels of PSD95, phosphorylated AKT and phosphorylated ERK were decreased after LY294002 injection in CM rats compared to CM + DMSO rats. There were no significant changes in the total ERK and AKT protein levels. One-way ANOVA with Dunnett’s post hoc test; n = 6/group; **P < 0.01, ***P < 0.001 vs. the Sham + DMSO group; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. the CM + DMSO group