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Fig. 5 | The Journal of Headache and Pain

Fig. 5

From: P2X7R/NLRP3 signaling pathway-mediated pyroptosis and neuroinflammation contributed to cognitive impairment in a mouse model of migraine

Fig. 5

Inhibition of P2X7R attenuated IS-induced NLRP3 inflammasome activation and pyroptosis. (a-b) Representative immunoblots and quantification showed decreased expression levels of P2X7R, NLRP3 (inflammasome receptor) and cleaved caspase-1 (marker of inflammasome activation) in the cerebral cortex of IS-BBG mice compared to IS-VEH mice (n = 6 mice per group). (c-d) Representative immunoblots and quantification showed decreased expression levels of GSDMD-NT (pyroptotic marker) in the cerebral cortex of IS-BBG mice compared to IS-VEH mice (n = 6 mice per group). (ef) ELISA results showed decreased release of IL-1β and IL-18 (direct downstream products of inflammasome activation) in the cerebral cortex of IS-BBG mice compared to IS-VEH mice (n = 4 mice per group). *p < 0.05, **p < 0.01 and ***p < 0.001 as compared to sham-VEH mice; +p < 0.05, ++p < 0.01 and +++p < 0.001 as compared to IS-VEH mice. One-way ANOVA with Tukey’s multiple comparisons or Kruskal–Wallis with Dunn’s multiple comparisons test were performed for statistical analysis. Data are represented as mean ± SEM. Abbreviations: IS, inflammatory soup; BBG, brilliant blue G; VEH, vehicle; GSDMD-NT, N-terminal Gasdermin D; ELISA, enzyme-linked immunosorbent assay

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