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Fig. 2 | The Journal of Headache and Pain

Fig. 2

From: P2X7R/NLRP3 signaling pathway-mediated pyroptosis and neuroinflammation contributed to cognitive impairment in a mouse model of migraine

Fig. 2

Repeated dural IS stimulation upregulated P2X7R in the cortical and hippocampal neurons. (a-b) Representative immunoblots and quantification illustrated increased levels of P2X7R in the cerebral cortex and hippocampus after repeated dural IS stimulation. (c-e) Double immunofluorescence staining showed that P2X7R was co-localized within neurons (NeuN positive) in the cerebral cortex and hippocampus. No substantial colocalization of P2X7R was observed in microglia (Iba-1 positive) and astrocytes (GFAP positive) in the cortex and hippocampus. Magnification × 40. Insets show a higher magnification view (Zoom). Scale bar, 20 μm. Images were taken under identical exposures and conditions. n = 6 mice per group. *p < 0.05, **p < 0.01 and ***p < 0.001 as compared to sham mice. Datasets in (b) were analyzed using Student’s t test. Data are represented as mean ± SEM. Abbreviations: IS, inflammatory soup; NeuN, neuronal nuclei; Iba-1, ionized calcium-binding adaptor molecule-1; GFAP, glial fibrillary acidic protein 

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