Fig. 5

AZD8797 treatment attenuates SE-induced increases in nociceptive behaviour, microglial activation, and protein changes. A Flow chart of the experimental groups: sham-PBS, comorbidity-PBS, and comorbidity-AZD. B Mechanical threshold in the comorbidity-PBS group was decreased compared to that in the sham-PBS group and was reversed in the comorbidity-AZD group. Mean ± SEM; one-way ANOVA; n = 6 rats per group; ***P < 0.001. C Head-scratching counts were increased in the comorbidity-PBS group compared to the sham-PBS group, and were restored after AZD8797 treatment. Mean ± SEM; one-way ANOVA; n = 6 rats per group; ***P < 0.001. D Immunofluorescence staining of iba1 showing that AZD8797 reversed epileptic microglial activation and hyperplasia. Scale bar = 20 μm. The white box was enlarged. E Representative western blot images showing CX3CR1/FKN/proBDNF/BDNF/iba1 protein expression in the sp5c of the different groups. F Quantification of proBDNF and BDNF protein levels in the sham-PBS group, comorbidity-PBS group, and comorbidity-AZD group. Mean ± SEM; one-way ANOVA; n = 3 rats per group; *P < 0.05, **P < 0.01