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Fig. 3 | The Journal of Headache and Pain

Fig. 3

From: FKN/CX3CR1 axis facilitates migraine-Like behaviour by activating thalamic-cortical network microglia in status epilepticus model rats

Fig. 3

SE upregulates FKN/CX3CR1 in the sp5c of migraine model rats. A Representative western blot images showing CX3CR1 and FKN protein expression in the cortex/thalamus/sp5c of SE model rats. B-C Western blot analysis showing that CX3CR1 and FKN protein levels of in the cortex/thalamus/sp5c were upregulated in the SE group compared to the sham group. Mean ± SEM; unpaired t test versus sham group; n = 3 rats per group; *P < 0.05, **P < 0.01. D Representative western blots showing CX3CR1 and FKN in the different groups. (E) Western blot analysis showing that the protein levels of CX3CR1/FKN were increased in the migraine group, and further increased in the comorbidity group. Mean ± SEM; one-way ANOVA; n = 4 rats per group; *P < 0.05, **P < 0.01, ***P < 0.001. F Representative immunofluorescence images showing CX3CR1 + cells (green) colocalized with Iba1 + cells (red) in the sp5c of different groups. Scale bars = 50 μm. The white box was enlarged. G Representative immunofluorescence images showing FKN + cells (green) colocalized with Neun + cells (red) in the sp5c in the different groups. Scale bars = 50 μm. H-I Quantification of CX3CR1 and iba1 immunoreactivity (in arbitrary units) in the sp5c. Mean ± SEM; one-way ANOVA; n = 3 rats per group; **P < 0.01, ***P < 0.001. J Quantification of FKN immunoreactivity (in arbitrary units) in the sp5c. Mean ± SEM; one-way ANOVA; n = 3 rats per group; **P < 0.01, ***P < 0.001

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