Fig. 2

SE-induced increase in microglial activation and number in the cortex/thalamus/sp5c in adult rats. A Representative immunohistochemical staining of iba1 in the cortex, PV, LDVL, VM, and sp5c. Scale bars = 100 μm. B Iba1-positive (iba1 +) areas in different brain regions. C Quantification of the number of Iba1 + cells. Mean ± SEM; unpaired t test versus sham group; n = 3 rats per group. *P < 0.05, ***P < 0.001 compared to the sham group. D-E Sholl analysis of microglia showed that SE-induced branch shortening and increased synapses compared to those in the sham group. Mean ± SEM; unpaired t test versus sham group; n = 6–11 cells per group; **P < 0.01. F Western blot analysis of iba1 in the cortex, thalamus, and sp5c. G Western blot showing that the protein levels of iba1 in the cortex/-thalamus/sp5c were increased compared those in to the sham group. n = 3 rats per group. Mean ± SEM; unpaired t test versus sham group; *P < 0.05, **P < 0.01, ***P < 0.001 compared to the sham group