Table 1 Antibodies used in western blot and immunofluorescence staining analysis
Antibody | Manufacturer | Host | Dilution |
|---|---|---|---|
For Wertern blot analysis | |||
  CGRP | Abcam, UK | Rabbit | 1:3000 |
  PKC | Bioss, China | Rabbit | 1:1000 |
  PSD95 | Cell signaling technology, USA | Mouse | 1:1000 |
  Synaptophysin | Abcam, UK | Rabbit | 1:20000 |
  Synaptotagmin-1 | Bioss, China | Rabbit | 1:1000 |
  p-ERK | Cell signaling technology, USA | Rabbit | 1:2000 |
  ERK | Cell signaling technology, USA | Rabbit | 1:1000 |
  p-CREB-133 | Abcam, UK | Rabbit | 1:5000 |
  CREB | Wanleibio, China | Rabbit | 1:1000 |
  GAPDH | ZEN BIO, China | Mouse | 1:8000 |
  β-actin | ZSGB BIO, China | Mouse | 1:1500 |
  Anti-rabbit IgG (HRP) | ZEN BIO, China | Goat | 1:5000 |
  Anti-mouse IgG (HRP) | ZEN BIO, China | Goat | 1:5000 |
For Immunofluorescent staining | |||
  CGRP | Santa Cruz, USA | Mouse | 1:100 |
  CLR | Bioss, China | Rabbit | 1:1000 |
  RAMP1 | Proteintech, China | Rabbit | 1:200 |
  c-Fos | Novus Biologicals, USA | Rabbit | 1:5000 |
  NeuN | Abcam, UK | Mouse | 1:500 |
  GFAP | Santa Cruz, USA | Mouse | 1:200 |
  Iba-1 | ThermoFisher, USA | Mouse | 1:500 |
  Cy3-conjugatedgoat anti-rabbit IgG | Beyotime, China | Goat | 1:500 |
  Alexa Fluor 488 goat anti-mouse IgG | Beyotime, China | Goat | 1:500 |