Fig. 5
BIBN4096BS treatment regulated the PKC/ERK/CREB signal and attenuated neuronal activation in VN after CM. A Representative western blot bands and densitometric quantification of CGRP in VN. n = 6/group. Representative micrographs (B) and quantitative analysis (C) of CLR and RAMP1 positive staining cells in VN. n = 6/group. D The mRNA levels of CLR and RAMP1 in VN. n = 6/group. Representative western blot bands and densitometric quantification of PKC (E), p-ERK (F) and p-CREB-S133 (G) in VN after CM. n = 6/group. The white dotted line frame indicated the VN area used for analysis (H). Representative micrographs (I) and quantitative analysis (J) of c-Fos staining in VN. The nuclei were stained with DAPI (blue). n = 6/group. Results are mean ± SD. FOV = 1.02 × 106 µm3. One-way ANOVA, Dunnett; *p < 0.05 compared with saline group, #p < 0.05 compared with CM + vehicle group. 4 V: the fourth ventricle