Fig. 2
Increased expression levels of calcitonin gene-related peptide (CGRP) and its receptor components in the vestibular nucleus (VN) after CM. A Representative micrographs and quantitative analysis of CGRP positive (green) fibers in the trigeminal nucleus caudalis (TNC). n = 6/group. B Representative western blots bands and densitometric quantification of CGRP in VN. n = 6/group. C Schematic illustration of brain tissue shows the VN region depicted with a white dotted line. Representative micrographs (D) and quantitative analysis (E) of calcitonin receptor-like receptor (CLR) and receptor activity modifying protein 1 (RAMP1) in VN. n = 6/group. F The mRNA levels of CLR and RAMP1 in VN. n = 6/group. G H Representative microphotographs of co-immunofluorescence staining of CLR (red) and RAMP1 (red) with astrocytes (GFAP, green), microglia (Iba-1, green), and neurons (NeuN, green) in VN after CM. The nuclei were stained with DAPI (blue). n = 3/group. Results are mean ± SD. FOV = 1.02 × 106 µm3. One-way ANOVA, Dunnett; *p < 0.05 compared with saline group. 4 V: the fourth ventricle