Fig. 3

Effects of ACEA on K⁺-induced CGRP release from dura and TG in the presence of AMG9810. A Application of 140 nM ACEA in the presence of AMG9810, did not cause a significant increase in CGRP release from the dura (n = 12). KCl (60 mM K⁺) caused a significant increase in CGRP release, which in the presence of 1 μM AMG9810 was significantly attenuated by ACEA. B Graphical illustration of the sample pairing. C Application of 140 nM ACEA had no effect on the CGRP release from the TG per se, but significantly inhibited the release induced by 60 mM K⁺ (n = 12) in the presence of 1 μM AMG9810. D Graphical illustration of the sample pairing. B = baseline. Timeline in minutes are added on the lower panel. Data are shown as mean ± SEM or their individual data points with pairing, and with p values obtained with Student’s T-test being depicted in the graph (* = p < 0.05). For reference 100 pg/ml equals 26.4 pmol/l