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Table 1 All antibodies used in western blotting and immunofluorescence staining

From: Sphingosine-1 phosphate receptor 1 contributes to central sensitization in recurrent nitroglycerin-induced chronic migraine model

Antibody Manufacturer Host Dilution
For western blotting analysis
 S1PR1 Abcam, UK Rabbit 1:1000
 pSTAT3 Cell Signaling Technology, USA Rabbit 1: 2000
 STAT3 Cell Signaling Technology, USA Mouse 1: 1000
 iNOS Proteintech, China Rabbit 1: 1000
 GAPDH ZEN-BIOSCIENCE, China Mouse 1: 5000
 Anti-mouse IgG (HRP) ZEN-BIOSCIENCE, China Goat 1: 9000
 Anti-rabbit IgG (HRP) ZEN-BIOSCIENCE, China Goat 1: 5000
For immunofluorescence staining
 S1PR1 Abcam, UK Rabbit 1: 400
 Iba1 Wako, Japan Rabbit 1: 400
 Iba1 Wako, Japan Goat 1: 1000
 NeuN Cell Signaling Technology, USA Mouse 1: 400
 GFAP Santa Cruz, USA Mouse 1: 200
 CGRP Santa Cruz, USA Mouse 1: 100
 c-fos Novus Biologicals, USA Rabbit 1: 5000
 Alexa Fluor 488 goat anti-mouse IgG Beyotime, China Goat 1: 500
 Cy3-conjugated goat anti-rabbit IgG Beyotime, China Goat 1: 500
 DyLight 488 rabbit anti-goat IgG Abbkine, USA Rabbit 1: 500