Table 1 All antibodies used in western blotting and immunofluorescence staining
Antibody | Manufacturer | Host | Dilution |
|---|---|---|---|
For western blotting analysis | |||
 S1PR1 | Abcam, UK | Rabbit | 1:1000 |
 pSTAT3 | Cell Signaling Technology, USA | Rabbit | 1: 2000 |
 STAT3 | Cell Signaling Technology, USA | Mouse | 1: 1000 |
 iNOS | Proteintech, China | Rabbit | 1: 1000 |
 GAPDH | ZEN-BIOSCIENCE, China | Mouse | 1: 5000 |
 Anti-mouse IgG (HRP) | ZEN-BIOSCIENCE, China | Goat | 1: 9000 |
 Anti-rabbit IgG (HRP) | ZEN-BIOSCIENCE, China | Goat | 1: 5000 |
For immunofluorescence staining | |||
 S1PR1 | Abcam, UK | Rabbit | 1: 400 |
 Iba1 | Wako, Japan | Rabbit | 1: 400 |
 Iba1 | Wako, Japan | Goat | 1: 1000 |
 NeuN | Cell Signaling Technology, USA | Mouse | 1: 400 |
 GFAP | Santa Cruz, USA | Mouse | 1: 200 |
 CGRP | Santa Cruz, USA | Mouse | 1: 100 |
 c-fos | Novus Biologicals, USA | Rabbit | 1: 5000 |
 Alexa Fluor 488 goat anti-mouse IgG | Beyotime, China | Goat | 1: 500 |
 Cy3-conjugated goat anti-rabbit IgG | Beyotime, China | Goat | 1: 500 |
 DyLight 488 rabbit anti-goat IgG | Abbkine, USA | Rabbit | 1: 500 |