|
For western blotting analysis
|
|
S1PR1
|
Abcam, UK
|
Rabbit
|
1:1000
|
|
pSTAT3
|
Cell Signaling Technology, USA
|
Rabbit
|
1: 2000
|
|
STAT3
|
Cell Signaling Technology, USA
|
Mouse
|
1: 1000
|
|
iNOS
|
Proteintech, China
|
Rabbit
|
1: 1000
|
|
GAPDH
|
ZEN-BIOSCIENCE, China
|
Mouse
|
1: 5000
|
|
Anti-mouse IgG (HRP)
|
ZEN-BIOSCIENCE, China
|
Goat
|
1: 9000
|
|
Anti-rabbit IgG (HRP)
|
ZEN-BIOSCIENCE, China
|
Goat
|
1: 5000
|
|
For immunofluorescence staining
|
|
S1PR1
|
Abcam, UK
|
Rabbit
|
1: 400
|
|
Iba1
|
Wako, Japan
|
Rabbit
|
1: 400
|
|
Iba1
|
Wako, Japan
|
Goat
|
1: 1000
|
|
NeuN
|
Cell Signaling Technology, USA
|
Mouse
|
1: 400
|
|
GFAP
|
Santa Cruz, USA
|
Mouse
|
1: 200
|
|
CGRP
|
Santa Cruz, USA
|
Mouse
|
1: 100
|
|
c-fos
|
Novus Biologicals, USA
|
Rabbit
|
1: 5000
|
|
Alexa Fluor 488 goat anti-mouse IgG
|
Beyotime, China
|
Goat
|
1: 500
|
|
Cy3-conjugated goat anti-rabbit IgG
|
Beyotime, China
|
Goat
|
1: 500
|
|
DyLight 488 rabbit anti-goat IgG
|
Abbkine, USA
|
Rabbit
|
1: 500
|
