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Fig. 6 | The Journal of Headache and Pain

Fig. 6

From: Sphingosine-1 phosphate receptor 1 contributes to central sensitization in recurrent nitroglycerin-induced chronic migraine model

Fig. 6

Inhibition of STAT3 activity attenuated chronic NTG-induced mechanical and thermal hyperalgesia. A-C Representative immunoblot showed that the expression level of pSTAT3 was up-regulated after repeated NTG administration (A, B), while the expression of STAT3 remained unchanged (A, C). The band intensities of pSTAT3/STAT3 were compared with GAPDH, respectively. n = 6 per group. ***p < 0.001 compared with the Saline group. D, F Representative immunoblot showed that the expression level of pSTAT3 decreased in the NTG + W146 group, but the expression level of STAT3 remained unchanged. n = 6 per group. * p < 0.05 compared with the Saline group. # p < 0.05 compared with the NTG group. G-I The STAT3 signaling inhibitor AG490 reversed the increase of pSTAT3(G, H) after repeated NTG injection but did not affect STAT3(G, I) in the TCC area. n = 6 per group. ** p < 0.01 compared with the Saline group. ##p < 0.01 compared with the NTG group. J-L Baseline thermal and mechanical pain threshold response of mice. Two-way ANOVA and Bonferroni post hoc test analysis; *p < 0.05, **p < 0.01 and ***p < 0.001 compared with the Saline group; #p < 0.05, ##p < 0.01 and ###p < 0.001 for the NTG + AG490 group compared with the NTG group

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