Fig. 5

Blocking S1PR1 attenuates LPS-induced increase of pSTAT3 in BV2 microglia in vitro. A-C Representative western blots results showed that LPS induced an increase of pSTAT3 and this increase was time-dependent, most obvious after 12 h of LPS stimulation in BV2 cells (A, B); However, LPS did not change the expression of STAT3 (A, C); Data were presented as mean ± SEM of three independent experiments; *p < 0.05 compared with LPS 0 h. D-F Pre-incubation of W146 before LPS stimulation could reduce the expression of pSTAT3, but had no effect on STAT3. Data were presented as mean ± SEM of three independent experiments; ***p < 0.001 vs. Sham group; ^#p < 0.05, ^##p < 0.01 vs. LPS group