Skip to main content
Fig. 3 | The Journal of Headache and Pain

Fig. 3

From: Sphingosine-1 phosphate receptor 1 contributes to central sensitization in recurrent nitroglycerin-induced chronic migraine model

Fig. 3

Blocking S1PR1 signaling reduced the expression of CGRP and c-fos in the TCC. A, B Representative immunofluorescence staining images (A) and quantitative analysis of CGRP in different groups (B). Repeated daily injection of W146 reversed the increased immunoreactivity of CGRP induced by chronic NTG administration. n = 3/group; six sections from each mouse; *p < 0.05 vs. Saline group; ###p < 0.001 vs. NTG group. Scale bars = 50 μm. C, D Double immunofluorescence staining images of c-fos (red) and DAPI (blue) in the TCC area, these results indicated daily administration of W146 decreased c-fos ir cells. n = 3/group; ***p < 0.001 compared with the Saline group; ###p < 0.001 for the NTG + W146 group compared with the NTG group. Scale bars = 50 μm

Back to article page