Table 2 Details of primary and secondary antibodies
Primary antibodies | ||||
Antigen | Dilution | Species | Immunogen | Supplier |
CGRP (ab81887) | 1;100 | Mouse | Rat alpha-CGRP | Abcam, Cambridge, UK |
5-HT1B (ab13896) | 1;100 | Rabbit | Amino acids 8–26 and 263–278 of 5- HT1B | Abcam, Cambridge, UK |
5-HT1D (ab13895) | 1;100 | Rabbit | Amino acids 1–18 and 251–267 of rat 5-HT1D | Abcam, Cambridge, UK |
5-HT1F (SP4043P) | 1;100 | Rabbit | N-terminus extracellular domain of human 5-HT1F | Acris Antibodies, San Diego, CA, USA |
5-HT (20079) | 1;100 | Goat | Serotonin whole molecule conjugated to BSA with paraformaldehyde. | Immunostar, Hudson, WI, USA |
5-HT1F (TA340657) | 1;100 | Rabbit | 5-HT1F Receptor antibody was raised against synthetic 19 amino acid peptide from N-terminal extracellular domain of human 5HT1F Receptor | Origene, Rockville, MD, USA |
CASPR (MABN69) | 1;100 | Mouse | Recombinant protein corresponding to rat contactin associated protein 1. | EMD milipore Corp. Temecula, CA, USA |
RAMP1 (844) | 1;200 | Goat | C-terminal of human RAMP1 | Merck & Co, Inc., West Point, PA, USA. |
MBP (MA5-15922) | 1;100 | Mouse | Purified recombinant fragment of human MBP expressed in E.Coli. | Thermo Fisher Scientific, Waltham, MA, USA. |
Secondary antibodies | ||||
Product | Dilution | Immunogen | Supplier | |
FITC | 1;100 | Anti-rabbit | Cayman Chemical, Ann Arbor, MI, USA | |
Alexa flour 594 | 1;100 | Anti-mouse | Jackson Immunoresearch Laboratories, Inc., West Grove, PA, USA | |
Dyelight 488 | 1;100 | Anti-rabbit | Jackson Immunoresearch Laboratories, Inc., West Grove, PA, USA | |
Cy3 | 1;200 | Anti-goat | Jackson Immunoresearch Laboratories, Inc., West Grove, PA, USA | |
FITC | 1;100 | Anti-mouse | Jackson Immunoresearch Laboratories, Inc., West Grove, PA, USA | |