Fig. 1
5-HT1 receptor light emission measurement, from representative areas of 0.09375 mm2 in each slide (18 microphotographs for each antibody, n = 6). All microphotographs were taken in greyscale at 333 ms exposure in FITC filter. Each primary antibody (all rabbit, 1:100) was labelled with the same secondary antibody (FITC, anti-rabbit, 1:100). Measurements were performed in imageJ using the freehand tool to trace neuron areas. All data presented as mean ± 1 standard deviation. The data was further analysed with Kruskal Wallis non-parametric test with Dunn`s post-hoc test. A 5-HT1B (76.4 ± 11.1 AU) and 5-HT1D (52.8 ± 11.6 AU) were the most prominently emitted. 5-HT1F (23.6 ± 4.2 AU) did not significantly differ from the negative control (19.1 ± 7.2 AU). B No significant difference between the measured neuron areas was found