Fig. 2

Activation of Piezo1 channels has no impact on increased nociceptive firing during PregS application. (A) Time course of MUA in meningeal nerves of female mice when first 5 μM Yoda1 and then 50 μM PregS was applied (mean ± SEM, n = 7). (B) Time course of MUA in female mice when first 50 μM PregS and then 5 μM Yoda1 was applied (mean ± SEM, n = 5). (C) Histograms show the percentage of increased nociceptive firing in females during 10-min recordings following 50 μM PregS application when administered before (mean ± SEM, n = 5) and after 5 μM Yoda1 application (mean ± SEM, n = 7). Notice that there is no difference in the proportion of increased nociceptive firing between the different orders of drug application (p = 0.63, Mann–Whitney U test). (D) Histograms show the percentage of increased nociceptive firing in females during 10-min recordings following 5 μM Yoda1 application when administered before (mean ± SEM, n = 7) and after 50 μM PregS application (mean ± SEM, n = 5). Notice there is no difference in the fraction of increased nociceptive firing between the different orders of drug application (p = 0.53, Mann–Whitney U test)