Fig. 9
NTG-induced BBB alterations increased peripheral IL-17A access to the TNC. NTG administration increased IL-17A levels, while its transcription was not initiated in the TNC. (A and B) Content of IL-17A in the TNC and serum of rats with NTG-induced migraine. (C and D) The Ct value of the IL-17A mRNA in the TNC and TG of rats with NTG-induced migraine. NTG administration altered BBB permeability. (E) Bright-field image of EB permeation in rats with NTG-induced migraine. (F) EB permeability in the TNC of rats with NTG-induced migraine. (G) Images of EB staining in the TNC of rats with NTG-induced migraine. NTG administration did not induce CD4+/IL-17A+ T cell migration into the TNC. (H) Images of CD4 and IL-17A co-staining in rats with NTG-induced migraine. Statistical analysis was performed using one-way ANOVA, n = 3, *p < 0.05 compared to the control group