Fig. 4

Effect of OT on CGRP and c-fos expression levels. A and C show c-fos, B and D show CGRP. Immunofluorescence staining showed that the number of c-fos-positive cells per FOV and the average fluorescence intensity of CGRP were significantly increased in the NTG + vehicle group compared with the sham group. OT administration significantly reduced the expression of CGRP and c-fos, while these effects were prevented after adding L368,899 (scale bars: 20 μm for c-fos, 100 μm for CGRP). E, F and G WB data quantification showed similar results to the immunofluorescence staining results. One-way ANOVA with Dunnett’s post hoc test, n = 4/group for immunofluorescence analysis, n = 6/group for WB analysis; ***p < 0.001 vs. the sham group; ###p < 0.001 vs. the NTG + vehicle group; &&&p < 0.001 vs. the NTG + OT group