Fig. 2

The increase of OTR in CM mouse and the distribution of OTR in the TNC. A The white full line frame indicates the TNC area stained for OTR. B Representative images of OTR immunoreactivity in the sham and NTG groups. C The average number of OTR-positive cells in the NTG group was significantly increased compared to that in the sham group (scale bar: 20 μm). D and E WB analysis showed that OTR protein expression in the NTG group was significantly increased compared with that in the sham group. F The mRNA expression of OTR in the NTG group was statistically higher than that in the sham group. G Double immunofluorescence labeling of OTR (green) and NeuN (red). In the TNC, most OTR was distributed in neurons (scale bar: 50 μm). H Double immunofluorescence labeling of OTR (green) and PSD-95 (red). Some OTR was colocalized with the postsynaptic membrane in the TNC (scale bar: 50 μm). Two-tailed Student’s t-test, n = 4/group for immunofluorescence analysis, n = 6/group for WB analysis; **p < 0.01, ***p < 0.001 vs. the sham group