Fig. 6

Peripheral CGRP decreases pixel area of the eye (increases the squint response) during both the active and inactive phases. A Mean pixel area over time during the light phase (10 AM) for each baseline (5 min recording, no injection) and treatment (5 min recording, 30 min post injection) for all mice injected with either Veh (PBS, n = 16, top) or CGRP (0.1 mg/kg, n = 15, bottom). B Mean overall pixel area from panel A ± SEM for all mice during baseline (B) and treatment (Tx) conditions. Empty symbols represent females, and full symbols represent males. There were no statistical differences between males and females. Two-way (factors: time x treatment) ANOVA, Sidak’s multiple comparison test ***p < 0.001 compared to corresponding baseline. C Mean pixel area over time during the night phase (10 AM) for each baseline (5 min recording, no injection) and treatment (5 min recording, 30 min post injection) for all mice injected with either Veh (PBS, n = 16, top) or CGRP (0.1 mg/kg, n = 15, bottom). D Mean overall pixel area from panel C ± SEM for all mice during baseline (B) and treatment (Tx) conditions. Empty symbols represent females, and full symbols represent males. There were no statistical differences between males and females. Two-way (factors: time x treatment) ANOVA, Sidak’s multiple comparison test ***p < 0.001 compared to corresponding baseline