Fig. 4

Peripheral CGRP induces light aversion during both the active and inactive phases (reverse testing from Fig. 3). In all panels, grey areas represent the active phase of the animals when lights of the facility were turned off (night). Empty symbols represent females, and full symbols represent males. There were no statistical differences between males and females. A Experimental design: Mice were first baselined during their active phase (experiment starting at 8:00 pm), and then exposed to treatments on the first test day during their active phase (8 pm), and second test day during their inactive phase (experiment starting at 10:00 am). B Left panel. Time spent in the light zone by CD1 mice during sequential exposures to the light/dark chamber at 25,000 lx. On test days, animals were tested 30 min after injection of vehicle (PBS, i.p., n = 10) or CGRP (0.1 mg/kg i.p., n = 10). Two-way (factors: time x treatment) ANOVA, Sidak’s multiple comparison test *p < 0.05, **p < 0.01 and ***p < 0.001 compared to corresponding time point in vehicle group. Right panel. Mean time (± SEM) spent in the light zone per 5 min interval for individual mice during the same experiment represented in (C). Unpaired t-test **p < 0.01 and ***p < 0.001 comparing CGRP group with corresponding vehicle group