Fig. 1

Peripheral CGRP administration does not change the overall activity of mice throughout the circadian cycle but decreases activity during the first hour after administration. Distance traveled by CD1 mice was measured during 23 consecutive hours starting 30 min after injection of vehicle (PBS, i.p., n = 22) or CGRP (0.1 mg/kg i.p., n = 24), using LABORAS™. Each experiment was done a week apart over 3 consecutive weeks with the same animals. In all panels, grey areas represent the active phase of the animals when lights of the facility were turned off (night). Empty symbols represent females, and full symbols represent males. There were no statistical differences between males and females. A Schematics of the experimental protocol. B On week 1, CGRP was administered at 9:30 am and assay started at 10:00 am. Left panel shows the time course of distance travelled over 23 h. Two-way (factors: time x treatment) ANOVA (ns). The right panel shows the average of the distance traveled during the first 30 min of the week 1 test. Unpaired t-test **p = 0.0063 comparing CGRP with vehicle group. D On week 2, CGRP was administered at 1:30 pm and assay started at 2:00 pm. Left panel shows the time course of distance travelled over 23 h. Two-way (factors: time x treatment) ANOVA (ns). The right panel shows the average of the distance traveled during the first 30 min of the week 2 test. Unpaired t-test **p = 0.0035 comparing CGRP with vehicle group. F On week 3, CGRP was administered at 7:30 pm and assay started at 8:00 pm. Left panel shows the time course of distance travelled over 23 h. Two-way (factors: time x treatment) ANOVA (interaction and treatment factors ns). The right panel shows the average of the distance traveled during the first 30 min of the week 3 test. Unpaired t-test **p = 0.0042 comparing CGRP with vehicle group