Fig. 4

Representative example of the responses of optical parameters APC (panel a) and OIS (panel b) to three subsequent CCW electrical stimulations repeated every 10 min for one of the rats during the baseline stage. Each curve in panels a and b shows dynamics of APC and OIS parameters averaged for the selected two most representative ROIs. The red curve was obtained in the first trial (0 min), the blue in the second trial (10 min), and the black in the third trial (20 min). The box plots in the panels c and d show cumulative changes in the responses to the stimulation of APC and OIS, respectively. In the panels c and d, the response was estimated as an integral of the respective curve shown in graph a or b (see details in the text). The data were obtained every 10 min for all rats (n = 32) in the baseline stage (before pharmacological interventions)