Table 1 Details of the primary and secondary antibodies
Primary antibodies | ||||
Product | Host | Dilution | Immunogen | Company |
Oxytocin, cat No: AB911 | Rabbit, | 1:500 | Synthetic oxytocin (Sigma) conjugated to thyroglobulin, refs Giovannelli et al. 1990, Shen et al. 1992 | Millipore, Temecula, CA, USA |
Oxytocin, cat No: PA1–18416 | Guinea pig | 1:1000 | Synthetic peptide corresponding to the amino acids 20–28 from human Oxytocin-neurophysin 1, conjugated to carrier protein | Thermo Fisher Scientific, Rockford, IL, USA |
Oxytocin, cat No: orb185729 | Rabbit | 1:100 | KLC conjugated synthetic peptide derived between 30 and 18 amino acids of human Oxytocin | Biorbyt LLC, San Francisco, CA, USA |
Oxytocin receptor, product code: ab87312 | Goat | 1:400 | Synthetic peptide corresponding to the C terminal amino acids 355–367, ref. Jin Hwan Lee et al. 2019 | Abcam, Cambridge, UK |
Oxytocin receptor, cat No: AVR-013 | Rabbit | 1:100 | Peptide corresponding to amino acid residues 346–358 of rat Oxytocin receptor | Alomone Labs, Ltd., Jerusalem, Israel |
Oxytocin receptor, cat No: PA5–19038 | Goat | 1:50 | Synthetic peptide sequence corresponding to the C-terminus amino acids of OXTR, | Thermo Fisher Scientific, Rockford, IL, USA |
Oxytocin receptor, aa43–129, cat No: LS-C373020 | Rabbit | 1:100 | Antibody raised against rat recombinant oxytocin receptor (Val43-Leu129) | LifeSpan Biosciences, Nordic BioSite AB, Täby, SE |
CGRP (B47–1) | Rabbit | 1:1500 | Rat CGRP | Europroxima, Arnheim, Netherlands |
RAMP1 844 | Goat | 1:100 | C-terminal of human RAMP1 | Merck & Co., Inc., USA |
Secondary antibodies | ||||
Product | Dilution | Immunogen | Company | |
Cy2 | 1:100 | Anti-rabbit | Jackson Immunoresearch, PA, USA | |
FITC | 1:100 | Anti-rabbit | Cayman Chemical, Ann Arbor, MI, USA | |
Alexa 594 | 1:100 | Anti-rabbit | Jackson Immunoresearch, PA, USA | |
Cy3 | 1:100 | Anti-rabbit | Jackson Immunoresearch, PA, USA | |
Cy2 | 1:100 | Anti-goat | Jackson Immunoresearch, PA, USA | |
Cy3 | 1:400 | Anti-goat | Jackson Immunoresearch, PA, USA | |
Alexa 488 | 1:100 | Anti-guinea pig | Jackson Immunoresearch, PA, USA | |