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Table 1 Details of the primary and secondary antibodies

From: The distribution of oxytocin and the oxytocin receptor in rat brain: relation to regions active in migraine

Primary antibodies
ProductHostDilutionImmunogenCompany
Oxytocin, cat No: AB911Rabbit,1:500Synthetic oxytocin (Sigma) conjugated to thyroglobulin, refs Giovannelli et al. 1990, Shen et al. 1992Millipore, Temecula, CA, USA
Oxytocin, cat No: PA1–18416Guinea pig1:1000Synthetic peptide corresponding to the amino acids 20–28 from human Oxytocin-neurophysin 1, conjugated to carrier proteinThermo Fisher Scientific, Rockford, IL, USA
Oxytocin, cat No: orb185729Rabbit1:100KLC conjugated synthetic peptide derived between 30 and 18 amino acids of human OxytocinBiorbyt LLC, San Francisco, CA, USA
Oxytocin receptor, product code: ab87312Goat1:400Synthetic peptide corresponding to the C terminal amino acids 355–367, ref. Jin Hwan Lee et al. 2019Abcam, Cambridge, UK
Oxytocin receptor, cat No: AVR-013Rabbit1:100Peptide corresponding to amino acid residues 346–358 of rat Oxytocin receptorAlomone Labs, Ltd., Jerusalem, Israel
Oxytocin receptor, cat No: PA5–19038Goat1:50Synthetic peptide sequence corresponding to the C-terminus amino acids of OXTR,Thermo Fisher Scientific, Rockford, IL, USA
Oxytocin receptor, aa43–129, cat No: LS-C373020Rabbit1:100Antibody raised against rat recombinant oxytocin receptor (Val43-Leu129)LifeSpan Biosciences, Nordic BioSite AB, Täby, SE
CGRP (B47–1)Rabbit1:1500Rat CGRPEuroproxima, Arnheim, Netherlands
RAMP1 844Goat1:100C-terminal of human RAMP1Merck & Co., Inc., USA
Secondary antibodies
ProductDilutionImmunogenCompany
Cy21:100Anti-rabbitJackson Immunoresearch, PA, USA
FITC1:100Anti-rabbitCayman Chemical, Ann Arbor, MI, USA
Alexa 5941:100Anti-rabbitJackson Immunoresearch, PA, USA
Cy31:100Anti-rabbitJackson Immunoresearch, PA, USA
Cy21:100Anti-goatJackson Immunoresearch, PA, USA
Cy31:400Anti-goatJackson Immunoresearch, PA, USA
Alexa 4881:100Anti-guinea pigJackson Immunoresearch, PA, USA