Fig. 5

Oxytocin immunohistochemistry in the cerebellum and the Mesencephalic trigeminal nucleus. a. Oxytocin immunoreactivity was found in fibers in the cerebellar white matter and to some extend in the granular cell layer. No oxytocin positivity was observed in the Purkinje cell layer (PC) or the molecular layer. Insert: a low magnification image of cerebellar lobes. X indicates where the large magnification image is selected. b. In the Mesencephalic trigeminal nucleus (Me5), intense oxytocin immunoreactivity was found. In addition, surrounding the Me5 positive slender fibers were demonstrated. Insert: Higher magnification of Me5. c. The lower row shows in comparison RAMP1 immunoreactivity [25]. The staining of the white matter agrees with the one seen in the oxytocin staining. However, RAMP1 is also found in PC, which is not the case with oxytocin. d. RAMP1 staining showed distinct neuronal cytoplasmatic staining and, in addition, thick fiber immunoreactivity