Fig. 4

Oxytocin immunohistochemistry of the cerebral cortex. a. Thin fibers of layers III-VI of the motor cortex displayed oxytocin immunoreactivity. b and c. These fibers spanned through the layers in a delicate and well-defined pattern. Layer III (b) and layers V-VI (c) are shown in a higher magnification. d. As a comparison, the calcitonin gene-related peptide (CGRP) receptor component (receptor activity-modifying protein 1) RAMP1 is shown in the right panel. These results have been published earlier [25]. We showed that RAMP1 positive fibers spanned through the cortical layers, but here layer II positivity was found. In addition to the transversal positive fibers, RAMP1 positive horizontal fibers were revealed