Table 2 Antibodies used in Western blot analysis and immunofluorescent staining
Antibody | Manufacturer | Dilution |
|---|---|---|
For Western blot analysis | ||
 β-actin | Proteintech, USA | 1:3000 |
 GAPDH | Abways Technology, China | 1:5000 |
 mGluR5 | Milipore,USA | 1:3000 |
 CGRP | Abcam, UK | 1:3000 |
 PSD95 | Abcam, UK | 1:1000 |
 Synaptophysin | Abcam, UK | 1:5000 |
 PKC | Abcam, UK | 1:3000 |
 NR2B | Proteintech, USA | 1:1000 |
 p-NR2B-Y1472 | Bioss, China | 1:500 |
 CREB | Abcam, UK | 1:1000 |
 p-CREB-S133 | Abcam, UK | 1:3000 |
 c-Fos | Novus Biologicals, USA | 1:3000 |
 Anti-rabbit IgG (HRP) | Bioss, China | 1:9000 |
 Anti-mouse IgG (HRP) | Zhongshan Golden Bridge Bio, China | 1:5000 |
For Immunofluorescent Staining | ||
 CGRP | Santa Cruz, USA | 1:100 |
 Synaptotagmin-1 | Bioss, China | 1:200 |
 c-Fos | Synaptic Systems, Germany | 1:800 |
 Cy3-conjugated goat anti-rabbit IgG | Beyotime, China | 1:500 |
 Alexa Fluor 488 goat anti-rabbit IgG | Beyotime, China | 1:500 |
 Alexa Fluor 488 goat anti-mouse IgG | Beyotime, China | 1:500 |