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Table 1 Details of primary and secondary antibodies

From: Estrogen receptors α, β and GPER in the CNS and trigeminal system - molecular and functional aspects

Antigen

Dilution

Species

Immunogen

Supplier

Primary antibodies

 CGRP (PA1–36017)

1:500

guinea pig

Synthetic human CGRP

Thermo Scientific, IL, USA

 Estrogen Receptor α (H-184, sc-7207)

1:100

rabbit

Amino acids 2–185 of ER α of human origin

Santa Cruz Biotechnology, CA, USA

 Estrogen Receptor α (ab75635)

1:100

rabbit

Synthetic peptide corresponding to Human Estrogen Receptor alpha (N terminal).

Abcam, Cambridge, UK

 Estrogen Receptor β (PA1–311)

1:100

rabbit

Synthetic peptide corresponding to residues A(55)EPQKSPWCEARSLEH(70) of rat ER beta

Thermo Scientific, IL, USA

 Estrogen Receptor β (ab288)

1:250

rabbit

Recombinant 6-His fusion protein encoding amino acids 1–153 of human ER expressed in E.coli

Abcam, Cambridge, UK

 GPER (G protein-coupled estrogen receptor 1)

1:100

rabbit

(C) ETFRDKLRLYVAQK, corresponding to amino acid residues 329–342

Alomone Labs, Ltd., Jerusalem, Israel

 CASPR (contactin associated protein 1)

1:100

mouse

Recombinant protein corresponding to rat Caspr

Millipore Corporation, Temecula, CA, USA.

Secondary antibodies

 Anti-guinea pig

1:100

Alexa 488

Thermo Scientific, IL, USA

 Anti-guinea pig

1:400

Cy3

Jackson Immunoresearch Laboratories, West Grove, PA, USA

 Anti-rabbit

1:400

Cy3

Jackson Immunoresearch Laboratories, West Grove, PA, USA

 Anti-rabbit

1:100

Cy2

Jackson Immunoresearch Laboratories, West Grove, PA, USA

 Anti-mouse

1:400

Cy3

Jackson Immunoresearch Laboratories, West Grove, PA, USA