From: Estrogen receptors α, β and GPER in the CNS and trigeminal system - molecular and functional aspects
Antigen | Dilution | Species | Immunogen | Supplier |
---|---|---|---|---|
Primary antibodies | ||||
CGRP (PA1–36017) | 1:500 | guinea pig | Synthetic human CGRP | Thermo Scientific, IL, USA |
Estrogen Receptor α (H-184, sc-7207) | 1:100 | rabbit | Amino acids 2–185 of ER α of human origin | Santa Cruz Biotechnology, CA, USA |
Estrogen Receptor α (ab75635) | 1:100 | rabbit | Synthetic peptide corresponding to Human Estrogen Receptor alpha (N terminal). | Abcam, Cambridge, UK |
Estrogen Receptor β (PA1–311) | 1:100 | rabbit | Synthetic peptide corresponding to residues A(55)EPQKSPWCEARSLEH(70) of rat ER beta | Thermo Scientific, IL, USA |
Estrogen Receptor β (ab288) | 1:250 | rabbit | Recombinant 6-His fusion protein encoding amino acids 1–153 of human ER expressed in E.coli | Abcam, Cambridge, UK |
GPER (G protein-coupled estrogen receptor 1) | 1:100 | rabbit | (C) ETFRDKLRLYVAQK, corresponding to amino acid residues 329–342 | Alomone Labs, Ltd., Jerusalem, Israel |
CASPR (contactin associated protein 1) | 1:100 | mouse | Recombinant protein corresponding to rat Caspr | Millipore Corporation, Temecula, CA, USA. |
Secondary antibodies | ||||
Anti-guinea pig | 1:100 | Alexa 488 | Thermo Scientific, IL, USA | |
Anti-guinea pig | 1:400 | Cy3 | Jackson Immunoresearch Laboratories, West Grove, PA, USA | |
Anti-rabbit | 1:400 | Cy3 | Jackson Immunoresearch Laboratories, West Grove, PA, USA | |
Anti-rabbit | 1:100 | Cy2 | Jackson Immunoresearch Laboratories, West Grove, PA, USA | |
Anti-mouse | 1:400 | Cy3 | Jackson Immunoresearch Laboratories, West Grove, PA, USA |