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Table 1 Details of primary and secondary antibodies

From: Estrogen receptors α, β and GPER in the CNS and trigeminal system - molecular and functional aspects

Antigen Dilution Species Immunogen Supplier
Primary antibodies
 CGRP (PA1–36017) 1:500 guinea pig Synthetic human CGRP Thermo Scientific, IL, USA
 Estrogen Receptor α (H-184, sc-7207) 1:100 rabbit Amino acids 2–185 of ER α of human origin Santa Cruz Biotechnology, CA, USA
 Estrogen Receptor α (ab75635) 1:100 rabbit Synthetic peptide corresponding to Human Estrogen Receptor alpha (N terminal). Abcam, Cambridge, UK
 Estrogen Receptor β (PA1–311) 1:100 rabbit Synthetic peptide corresponding to residues A(55)EPQKSPWCEARSLEH(70) of rat ER beta Thermo Scientific, IL, USA
 Estrogen Receptor β (ab288) 1:250 rabbit Recombinant 6-His fusion protein encoding amino acids 1–153 of human ER expressed in E.coli Abcam, Cambridge, UK
 GPER (G protein-coupled estrogen receptor 1) 1:100 rabbit (C) ETFRDKLRLYVAQK, corresponding to amino acid residues 329–342 Alomone Labs, Ltd., Jerusalem, Israel
 CASPR (contactin associated protein 1) 1:100 mouse Recombinant protein corresponding to rat Caspr Millipore Corporation, Temecula, CA, USA.
Secondary antibodies
 Anti-guinea pig 1:100 Alexa 488 Thermo Scientific, IL, USA
 Anti-guinea pig 1:400 Cy3 Jackson Immunoresearch Laboratories, West Grove, PA, USA
 Anti-rabbit 1:400 Cy3 Jackson Immunoresearch Laboratories, West Grove, PA, USA
 Anti-rabbit 1:100 Cy2 Jackson Immunoresearch Laboratories, West Grove, PA, USA
 Anti-mouse 1:400 Cy3 Jackson Immunoresearch Laboratories, West Grove, PA, USA