Fig. 1

CGRP plasma levels, and miR-382-5p and miR-34a-5p expression in peripheral blood mononuclear cells of subjects with episodic migraine and chronic migraine with medication overuse. EM: episodic migraine (n = 27); CM-MO: chronic migraine with medication overuse (n = 28). Panel a: comparison of CGRP between EM and CM-MO subjects. Panel b: comparison of miR-382-5p between EM and CM-MO subjects. Panel c: comparison of miR-34a-5p between EM and CM-MO subjects. Box-plots: the distance between the top and bottom of the box represents the interquartile range (IQR: 25th percentile to 75th percentile). The line inside the box represents the median value, while the cross inside the box represents the mean value. The upper and lower whiskers represent the maximum and minimum values respectively (outliers excluded). Black dots above the upper whiskers represent possible outliers (outliers are statistically defined as values outside the 75th percentile + 1.5*IQR). Δ: EM vs. CM-MOH: p < 0.005 (Mann-Whitney Test with post-hoc Bonferroni’s correction). RQ: Relative quantification: 2^−∆∆Ct = 2 − ^{(∆Ct gene − ∆Ct housekeeping gene)}; Ct: cycle threshold