Fig. 7

Immunofluorescence results for viral expression in the primary motor cortex (a-f) and CGRP neuron expression in the trigeminal ganglion (g-l). a, b, c Optogenetic viral expression in the motor cortex of TN-Opto animals. d, e, f Null viral expression in the motor cortex of TN-Null animals. a, d EYFP, b, e DAPI, and c, f Merge. Scale bar = 200 μm. g-l PBS and α-CGRP-injected and DAPI-stained trigeminal ganglion cells. g-i Presence of anti-CGRP antibody binding due to active state of α-CGRP neuron (arrow) within the trigeminal ganglion of PBS injected animal (j-l). α-CGRP injected and DAPI-stained trigeminal ganglion cells showing absence of CGRP neurons due to inhibition of α-CGRP neurons within the trigeminal ganglion. g, j CGRP, h, k DAPI, and i, l Merge. Scale bar = 100 μm