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Fig. 4 | The Journal of Headache and Pain

Fig. 4

From: Neuropeptide FF receptor 2 inhibits capsaicin-induced CGRP Upregulation in mouse trigeminal ganglion

Fig. 4

The trigeminal CGRP of NPFFR2 overexpressed Tg mice and the capsaicin-upregulated CGRP expression in NPFFR2 KO mice. a The level of CGRP in trigeminal ganglion of NPFFR2 Tg mice was measured by ELISA. Data are represented as the mean ± SEM and were analyzed by unpaired Student’s t-test. *p < 0.05 compared to the control (N = 9 per group). b WT and NPFFR2 knockout (KO) mice were intracisternally infused with capsaicin for 2 h, after which trigeminal ganglion was collected. CGRP protein level in the trigeminal ganglion was measured by immunofluorescence staining. The results are presented as percentage of CGRP-positive cell number. Data are represented as mean ± SEM and were analyzed by two-way ANOVA with Bonferroni’s multiple comparisons test. *p < 0.05, **p < 0.01, comparing WT and NPFFR2 KO mice (N = 5 in saline group, N = 4 in capsaicin group). c Immunofluorescence staining of CGRP in WT and NPFFR2 KO mice. Scale bar = 50 μm

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