Fig. 3

Npffr2 gene silencing prevents capsaicin-induced CGRP expression in the trigeminal ganglion of mice. One week after mice were intracisternally infused with Npffr2-a or control LV-shRNA, the animals were treated with capsaicin for 2 h to activate the trigeminovascular pathway. a Trigeminal Npffr2 gene silencing was measured by real-time PCR one week after LV-shRNA infusion. Data are represented as the mean ± SEM and were analyzed by unpaired Student’s t-test. *p < 0.05 compared to the control (N = 4 per group). b The CGRP protein level in the trigeminal ganglion were measured by immunofluorescence staining. The results are presented as percentage of CGRP-positive cell number. Data are represented as mean ± SEM and were analyzed by two-way ANOVA with Bonferroni’s multiple comparison test. ***p < 0.001, comparing capsaicin and saline treated groups. ^###p < 0.001, comparing control and Npffr2 LV-shRNA-treated groups (N = 5 per group). c Immunofluorescence staining of CGRP protein in LV-shRNA- and capsaicin-injected mice. Scale bar = 50 μm