Fig. 6

Inhibition of P2X4Rs attenuates ATP-evoked release and synthesis of BDNF, which depends on p38-MAPK. a Representative Western blot results of intracellular BDNF in cells pre-incubated with 5-BDBD (20 μM). b The histogram presents the quantitative analysis of BDNF protein. n = 6/group, One-way ANOVA, ***p < 0.001 versus PBS, ##p < 0.01 versus ATP. c Levels of BDNF in the supernatants of BV2 cells in different groups 120 min after drug treatment. n = 8/group, ***p < 0.001 versus PBS, #p < 0.05 versus NTG. d Effect of treatment with 5-BDBD on p38MAPK expression after ATP stimulation. Representative Western blot results of p38/p-p38MAPK in BV2 cells. e The histogram presents the quantitative analysis of p38/p-p38MAPK protein. n = 6/group, **p < 0.01 versus PBS, ##p < 0.01 versus ATP. f Effect of treatment with SB203580 on BDNF release after ATP stimulation. The histogram presents the levels of BDNF in the supernatants of BV2 cells. One-way ANOVA, ***p < 0.001 versus PBS, ###p < 0.001 versus ATP. g Immunofluorescence staining for BDNF (red) in BV2 cells at 120 min after drug treatments. Scale bar = 20 μm